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Hepatic FGF21 deletion does not alter circulating FGF21, insulin, or estrogen levels. ( A ) Basal insulin serum concentration (pg/mL). ( B ) Basal estrogen serum concentration (pg/mL). ( C ) Hepatic Fgf21 mRNA expression. ( D ) FGF21 serum levels (pg/mL). ( E ) Hepatic expression of Fgf21 receptor 4 ( Fgf21r4 ). All data are presented as mean ± SEM. ** p < 0.01 and p -values were determined by using a Student’s t -test. LoxP: n = 11; FKO: n = 9. For <t>ELISA:</t> LoxP: n = 10; FKO: n = 9.
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Hepatic FGF21 deletion does not alter circulating FGF21, insulin, or estrogen levels. ( A ) Basal insulin serum concentration (pg/mL). ( B ) Basal estrogen serum concentration (pg/mL). ( C ) Hepatic Fgf21 mRNA expression. ( D ) FGF21 serum levels (pg/mL). ( E ) Hepatic expression of Fgf21 receptor 4 ( Fgf21r4 ). All data are presented as mean ± SEM. ** p < 0.01 and p -values were determined by using a Student’s t -test. LoxP: n = 11; FKO: n = 9. For <t>ELISA:</t> LoxP: n = 10; FKO: n = 9.
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Hepatic FGF21 deletion does not alter circulating FGF21, insulin, or estrogen levels. ( A ) Basal insulin serum concentration (pg/mL). ( B ) Basal estrogen serum concentration (pg/mL). ( C ) Hepatic Fgf21 mRNA expression. ( D ) FGF21 serum levels (pg/mL). ( E ) Hepatic expression of Fgf21 receptor 4 ( Fgf21r4 ). All data are presented as mean ± SEM. ** p < 0.01 and p -values were determined by using a Student’s t -test. LoxP: n = 11; FKO: n = 9. For <t>ELISA:</t> LoxP: n = 10; FKO: n = 9.
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Hepatic FGF21 deletion does not alter circulating FGF21, insulin, or estrogen levels. ( A ) Basal insulin serum concentration (pg/mL). ( B ) Basal estrogen serum concentration (pg/mL). ( C ) Hepatic Fgf21 mRNA expression. ( D ) FGF21 serum levels (pg/mL). ( E ) Hepatic expression of Fgf21 receptor 4 ( Fgf21r4 ). All data are presented as mean ± SEM. ** p < 0.01 and p -values were determined by using a Student’s t -test. LoxP: n = 11; FKO: n = 9. For <t>ELISA:</t> LoxP: n = 10; FKO: n = 9.
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Hepatic FGF21 deletion does not alter circulating FGF21, insulin, or estrogen levels. ( A ) Basal insulin serum concentration (pg/mL). ( B ) Basal estrogen serum concentration (pg/mL). ( C ) Hepatic Fgf21 mRNA expression. ( D ) FGF21 serum levels (pg/mL). ( E ) Hepatic expression of Fgf21 receptor 4 ( Fgf21r4 ). All data are presented as mean ± SEM. ** p < 0.01 and p -values were determined by using a Student’s t -test. LoxP: n = 11; FKO: n = 9. For ELISA: LoxP: n = 10; FKO: n = 9.

Journal: International Journal of Molecular Sciences

Article Title: Hepatic FGF21 Deletion Improves Glucose Metabolism, Alters Lipogenic and Chrna4 Gene Expression, and Enhances Telomere Maintenance in Aged Female Mice

doi: 10.3390/ijms27010194

Figure Lengend Snippet: Hepatic FGF21 deletion does not alter circulating FGF21, insulin, or estrogen levels. ( A ) Basal insulin serum concentration (pg/mL). ( B ) Basal estrogen serum concentration (pg/mL). ( C ) Hepatic Fgf21 mRNA expression. ( D ) FGF21 serum levels (pg/mL). ( E ) Hepatic expression of Fgf21 receptor 4 ( Fgf21r4 ). All data are presented as mean ± SEM. ** p < 0.01 and p -values were determined by using a Student’s t -test. LoxP: n = 11; FKO: n = 9. For ELISA: LoxP: n = 10; FKO: n = 9.

Article Snippet: Serum 8-hydroxy-2-deoxyguanosine (8-OHdG), a marker of DNA oxidation, was quantified using the DNA Damage Competitive ELISA kit (EIADNAD, Thermo Fisher Scientific, Waltham, MA, USA).

Techniques: Concentration Assay, Expressing, Enzyme-linked Immunosorbent Assay

Gene expression changes related to lipid and glucose metabolism in the liver of FKO mice, along with serum adiponectin levels. ( A ) Relative mRNA levels of genes related to lipid metabolism: Pparα ( Peroxisome proliferator activated receptor alpha ), Hmgcs2 ( 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 ), Cd36 ( CD36 molecule ), Cpt1a ( Carnitine palmitoyltransferase 1a ), Chrebp ( MLX interacting protein-like ), Srebp1c ( Sterol regulatory element binding transcription factor 1 ), Fasn ( Fatty acid synthase ), Scd1 ( Stearoyl-Coenzyme A desaturase 1 ), Acox3 ( Acyl-Coenzyme A oxidase 3 ), Apoc2 ( Apolipoprotein C2 like ) and AdipoR2 ( Adiponectin Receptor 2 ). ( B ) Adiponectin serum levels (μg/mL). ( C ) Liver Triacylglycerol (TAG) levels (µmol/mg). ( D ) Total cholesterol serum levels (mg/dL). ( E ) Relative mRNA levels of genes related to gluconeogenesis: Pepck ( Phosphoenolpyruvate carboxykinase 1 ) and G6pc ( Glucose-6-phosphatase ); and glycolysis: Lpk ( Pyruvate kinase ) and Gluk ( Glucokinase ). All data are presented as mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001 and p -values were determined by using a Student’s t -test. LoxP: n = 10; FKO: n = 9. For adiponectin ELISA: LoxP: n = 8; FKO: n = 8.

Journal: International Journal of Molecular Sciences

Article Title: Hepatic FGF21 Deletion Improves Glucose Metabolism, Alters Lipogenic and Chrna4 Gene Expression, and Enhances Telomere Maintenance in Aged Female Mice

doi: 10.3390/ijms27010194

Figure Lengend Snippet: Gene expression changes related to lipid and glucose metabolism in the liver of FKO mice, along with serum adiponectin levels. ( A ) Relative mRNA levels of genes related to lipid metabolism: Pparα ( Peroxisome proliferator activated receptor alpha ), Hmgcs2 ( 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 2 ), Cd36 ( CD36 molecule ), Cpt1a ( Carnitine palmitoyltransferase 1a ), Chrebp ( MLX interacting protein-like ), Srebp1c ( Sterol regulatory element binding transcription factor 1 ), Fasn ( Fatty acid synthase ), Scd1 ( Stearoyl-Coenzyme A desaturase 1 ), Acox3 ( Acyl-Coenzyme A oxidase 3 ), Apoc2 ( Apolipoprotein C2 like ) and AdipoR2 ( Adiponectin Receptor 2 ). ( B ) Adiponectin serum levels (μg/mL). ( C ) Liver Triacylglycerol (TAG) levels (µmol/mg). ( D ) Total cholesterol serum levels (mg/dL). ( E ) Relative mRNA levels of genes related to gluconeogenesis: Pepck ( Phosphoenolpyruvate carboxykinase 1 ) and G6pc ( Glucose-6-phosphatase ); and glycolysis: Lpk ( Pyruvate kinase ) and Gluk ( Glucokinase ). All data are presented as mean ± SEM. * p < 0.05; ** p < 0.01; *** p < 0.001 and p -values were determined by using a Student’s t -test. LoxP: n = 10; FKO: n = 9. For adiponectin ELISA: LoxP: n = 8; FKO: n = 8.

Article Snippet: Serum 8-hydroxy-2-deoxyguanosine (8-OHdG), a marker of DNA oxidation, was quantified using the DNA Damage Competitive ELISA kit (EIADNAD, Thermo Fisher Scientific, Waltham, MA, USA).

Techniques: Gene Expression, Binding Assay, Enzyme-linked Immunosorbent Assay